{"id":431252,"date":"2024-10-20T07:27:11","date_gmt":"2024-10-20T07:27:11","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-23-30468623-dc-2023\/"},"modified":"2024-10-26T14:10:08","modified_gmt":"2024-10-26T14:10:08","slug":"bsi-23-30468623-dc-2023","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-23-30468623-dc-2023\/","title":{"rendered":"BSI 23\/30468623 DC 2023"},"content":{"rendered":"
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
---|---|---|---|---|---|---|---|
1<\/td>\n | 30468623-NC.pdf <\/td>\n<\/tr>\n | ||||||
3<\/td>\n | ISO_DIS17174ed1-id85543EnquiryPDF(en).pdf <\/td>\n<\/tr>\n | ||||||
6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 4 Principle 5 Reagents and materials 5.1 General 5.2 PCR reagents <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 6 Apparatus 7 Procedure 7.1 Sample preparation <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 7.2 DNA extraction 7.3 PCR 7.3.1 General 7.3.2 PCR setup <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | 7.3.3 Temperature-time program <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | 7.3.4 PCR controls 8 Evaluation 8.1 Evaluation of PCR products 8.2 Evaluation of the PCR results <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | 8.3 Sequencing of PCR products 8.4 Evaluation of sequence data 8.5 Comparison of the sequence with public databases 8.5.1 General <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | 8.5.2 Sequence comparison of cytb and\/or cox1 DNA sequences with GenBank 8.5.3 Sequence comparison of cox1 DNA sequences with BOLD <\/td>\n<\/tr>\n | ||||||
19<\/td>\n | 9 Interpretation of database query results <\/td>\n<\/tr>\n | ||||||
20<\/td>\n | 10 Validation status and performance criteria 10.1 Collaborative study for the identification of fish species based on cytb sequence analysis <\/td>\n<\/tr>\n | ||||||
21<\/td>\n | 10.2 Collaborative study for the identification of fish species based on cox1 sequence analysis <\/td>\n<\/tr>\n | ||||||
22<\/td>\n | 11 Test report <\/td>\n<\/tr>\n | ||||||
23<\/td>\n | Annex\u20acA (informative) Practical laboratory experiences with the amplifiability of cytb and cox1 segments from tested fish species <\/td>\n<\/tr>\n | ||||||
26<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" BS EN ISO 17174. Molecular biomarker analysis. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments<\/b><\/p>\n |