BSI 23/30468623 DC 2023
$13.70
BS EN ISO 17174. Molecular biomarker analysis. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments
Published By | Publication Date | Number of Pages |
BSI | 2023 | 27 |
PDF Catalog
PDF Pages | PDF Title |
---|---|
1 | 30468623-NC.pdf |
3 | ISO_DIS17174ed1-id85543EnquiryPDF(en).pdf |
6 | Foreword |
7 | Introduction |
9 | 1 Scope 2 Normative references 3 Terms and definitions |
12 | 4 Principle 5 Reagents and materials 5.1 General 5.2 PCR reagents |
13 | 6 Apparatus 7 Procedure 7.1 Sample preparation |
14 | 7.2 DNA extraction 7.3 PCR 7.3.1 General 7.3.2 PCR setup |
15 | 7.3.3 Temperature-time program |
16 | 7.3.4 PCR controls 8 Evaluation 8.1 Evaluation of PCR products 8.2 Evaluation of the PCR results |
17 | 8.3 Sequencing of PCR products 8.4 Evaluation of sequence data 8.5 Comparison of the sequence with public databases 8.5.1 General |
18 | 8.5.2 Sequence comparison of cytb and/or cox1 DNA sequences with GenBank 8.5.3 Sequence comparison of cox1 DNA sequences with BOLD |
19 | 9 Interpretation of database query results |
20 | 10 Validation status and performance criteria 10.1 Collaborative study for the identification of fish species based on cytb sequence analysis |
21 | 10.2 Collaborative study for the identification of fish species based on cox1 sequence analysis |
22 | 11 Test report |
23 | Annex€A (informative) Practical laboratory experiences with the amplifiability of cytb and cox1 segments from tested fish species |
26 | Bibliography |