| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
|---|---|---|---|---|---|---|---|
| 2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
| 4<\/td>\n | European Foreword Anchor 3 European Foreword to amendment A1 <\/td>\n<\/tr>\n | ||||||
| 5<\/td>\n | Blank Page <\/td>\n<\/tr>\n | ||||||
| 6<\/td>\n | Annex ZA (normative) Normative references to international publications with their relevant European publications <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | Blank Page <\/td>\n<\/tr>\n | ||||||
| 10<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
| 13<\/td>\n | 1 Scope 2 Normative references 3 Term and definition <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 4 Principle 5 Culture media, reagents and antisera 5.1 Enrichment medium: Modified tryptone soya broth with novobiocin (mTSB + N) 5.1.1 Modified tryptone soya broth (mTSB) 5.1.2 Novobiocin solution <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | Blank Page <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | 5.2 First selective isolation medium: Cefixime tellurite sorbitol MacConkey agar (CT-SMAC) 5.2.1 Base medium 5.2.2 Potassium tellurite solution <\/td>\n<\/tr>\n | ||||||
| 17<\/td>\n | 5.2.3 Cefixime solution 5.2.4 Complete medium 5.3 Second selective isolation medium <\/td>\n<\/tr>\n | ||||||
| 18<\/td>\n | 5.4 Nutrient agar 5.4.1 Composition 5.4.2 Preparation 5.4.3 Preparation of nutrient agar plates 5.5 Tryptone\/tryptophan medium 5.5.1 Composition 5.5.2 Preparation <\/td>\n<\/tr>\n | ||||||
| 19<\/td>\n | 5.6 Kovac\u2019s indole reagent 5.6.1 Composition 5.6.2 Preparation 5.7 Anti-Escherichia coli O157 immunomagnetic particles 5.8 Wash buffer: Modified phosphate buffer, 0,01mol\/l, of pH 7,2 5.8.1 Composition 5.8.2 Preparation 5.9 Normal saline solution 5.9.1 Composition <\/td>\n<\/tr>\n | ||||||
| 20<\/td>\n | Blank Page <\/td>\n<\/tr>\n | ||||||
| 21<\/td>\n | 5.9.2 Preparation 6 Apparatus and glassware 6.1 Apparatus for dry sterilization (oven) and\/or wet sterilization (autoclave) <\/td>\n<\/tr>\n | ||||||
| 22<\/td>\n | 6.16 Vortex mixer 7 Sampling 8 Preparation of test sample 9 Procedure (see annex A) 9.1 Test portion and initial suspension 9.2 Enrichment <\/td>\n<\/tr>\n | ||||||
| 23<\/td>\n | 9.3 Immunomagnetic separation (IMS) 9.3.1 General 9.3.2 Immunocapture 9.3.3 Separation 9.4 Plating out onto selective agars and identification of E. coli O157 colonies 9.4.1 Plating out <\/td>\n<\/tr>\n | ||||||
| 24<\/td>\n | 9.4.2 Recognition of typical E. coli O157 colonies 9.5 Confirmation 9.5.1 Selection of colonies 9.5.2 Biochemical confirmation: Indole formation 9.5.3 Serological identification <\/td>\n<\/tr>\n | ||||||
| 25<\/td>\n | 9.6 Further characterization 10 Quality assurance 10.1 Test strains for quality assurance purposes 10.2 Culture method 11 Expression of results \uea0112 Performance characteristics of the method 12.1 Interlaboratory study 12.2 Sensitivity <\/td>\n<\/tr>\n | ||||||
| 26<\/td>\n | 12.3 Specificity 12.4 LOD50 \uea0113 Test report <\/td>\n<\/tr>\n | ||||||
| 27<\/td>\n | Annex A (normative) Diagram of procedure <\/td>\n<\/tr>\n | ||||||
| 28<\/td>\n | \uea01Annex B (informative) Results of interlaboratory studies <\/td>\n<\/tr>\n | ||||||
| 31<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Microbiology of food and animal feeding stuffs. Horizontal method for the detection of Escherichia coli O157. Inclusion of performance testing of all culture media and reagent<\/b><\/p>\n |