{"id":338801,"date":"2024-10-19T23:42:47","date_gmt":"2024-10-19T23:42:47","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-156622018-tc\/"},"modified":"2024-10-25T22:49:13","modified_gmt":"2024-10-25T22:49:13","slug":"bs-en-156622018-tc","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-156622018-tc\/","title":{"rendered":"BS EN 15662:2018 – TC"},"content":{"rendered":"
This European Standard stipulates a method for the analysis of pesticide residues in foods of plant origin, such as fruits (including dried fruits), vegetables, cereals and many processed products thereof by using GC, GC-MS(\/MS), and\/or LC-MS(\/MS). The method has been collaboratively studied on a large number of commodity\/pesticide combinations. Precision data are summarized in FprCEN\/TR 17063. Guidelines for calibration are outlined in FprCEN\/TS 17061.<\/p>\n
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
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1<\/td>\n | compares BS EN 15662:2018 <\/td>\n<\/tr>\n | ||||||
2<\/td>\n | TRACKED CHANGES Text example 1 \u2014 indicates added text (in green) <\/td>\n<\/tr>\n | ||||||
166<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
170<\/td>\n | 1 Scope 2 Normative references 3 Principle 4 Preparation and storage of the samples 4.1 General 4.2 Laboratory sample <\/td>\n<\/tr>\n | ||||||
171<\/td>\n | 4.3 Partly-prepared test sample 4.4 Test sample 4.5 Test portion 5 Procedure <\/td>\n<\/tr>\n | ||||||
181<\/td>\n | 6 Evaluation of results 6.1 Identification and quantification 6.2 Calibration 6.3 Calculation of residue concentration <\/td>\n<\/tr>\n | ||||||
182<\/td>\n | 6.4 Validity of the method <\/td>\n<\/tr>\n | ||||||
188<\/td>\n | 7 Confirmatory tests 8 Precision 9 Test report <\/td>\n<\/tr>\n | ||||||
189<\/td>\n | Annex A (informative)Description of modules A.1 Reagents used in all extraction (E) and clean-up (C) and stabilization (S) modules <\/td>\n<\/tr>\n | ||||||
192<\/td>\n | A.2 Apparatus used in all extraction (E) and clean-up (C) and stabilization (S) modules A.3 Description of extraction (E) modules A.3.1 Module E1: Extraction of a 10 g test portion without additional water using acetonitrile A.3.1.1 Principle <\/td>\n<\/tr>\n | ||||||
193<\/td>\n | A.3.1.2 Procedure A.3.1.3 Calculation <\/td>\n<\/tr>\n | ||||||
194<\/td>\n | A.3.1.4 Flowchart of module E1 A.3.2 Module E2: Extraction of a 10 g test portion without additional water after addition of a) 0,6 ml or b) 0,2 ml of 5 mol\/l sodium hydroxide solution using acetonitrile A.3.2.1 Principle <\/td>\n<\/tr>\n | ||||||
195<\/td>\n | A.3.2.2 Procedure A.3.2.3 Calculation <\/td>\n<\/tr>\n | ||||||
196<\/td>\n | A.3.2.4 Flowchart of module E2 A.3.3 Module E3: Extraction of a 10 g test portion after addition of a) 2,5 ml or b) 4,5 ml of water using acetonitrile A.3.3.1 Principle <\/td>\n<\/tr>\n | ||||||
197<\/td>\n | A.3.3.2 Procedure A.3.3.3 Calculation <\/td>\n<\/tr>\n | ||||||
198<\/td>\n | A.3.3.4 Flowchart of module E3 A.3.4 Module E4: Homogenization of the sample with addition of water, extraction of a 13,5 g homogenate using acetonitrile A.3.4.1 Principle <\/td>\n<\/tr>\n | ||||||
199<\/td>\n | A.3.4.2 Procedure A.3.4.3 Calculation <\/td>\n<\/tr>\n | ||||||
200<\/td>\n | A.3.4.4 Flowchart of module E4 A.3.5 Module E5: Extraction of a 5 g test portion after addition of 10 ml of water using acetonitrile A.3.5.1 Principle <\/td>\n<\/tr>\n | ||||||
201<\/td>\n | A.3.5.2 Procedure A.3.5.3 Calculation <\/td>\n<\/tr>\n | ||||||
202<\/td>\n | A.3.5.4 Flowchart of module E5 A.3.6 Module E6: Extraction of a 5 g test portion after addition of 6 ml of water using acetonitrile A.3.6.1 Principle <\/td>\n<\/tr>\n | ||||||
203<\/td>\n | A.3.6.2 Procedure A.3.6.3 Calculation <\/td>\n<\/tr>\n | ||||||
204<\/td>\n | A.3.6.4 Flowchart of module E6 A.3.7 Module E7: Extraction of a 2 g test portion after addition of 10 ml of water using acetonitrile A.3.7.1 Principle <\/td>\n<\/tr>\n | ||||||
205<\/td>\n | A.3.7.2 Procedure A.3.7.3 Calculation <\/td>\n<\/tr>\n | ||||||
206<\/td>\n | A.3.7.4 Flowchart of module E7 A.3.8 Module E8: Extraction of a 10 g test portion without additional water with simultaneous alkaline hydrolysis of alkaline labile esters and conjugates using acetonitrile A.3.8.1 Principle <\/td>\n<\/tr>\n | ||||||
207<\/td>\n | A.3.8.2 Procedure A.3.8.3 Calculation <\/td>\n<\/tr>\n | ||||||
208<\/td>\n | A.3.8.4 Flowchart of module E8 A.3.9 Module E9: Extraction of 2 g or 5 g test portions after addition of water with simultaneous alkaline hydrolysis of alkaline labile esters and conjugates using acetonitrile A.3.9.1 Principle <\/td>\n<\/tr>\n | ||||||
209<\/td>\n | A.3.9.2 Procedure <\/td>\n<\/tr>\n | ||||||
210<\/td>\n | A.3.9.3 Calculation A.3.9.4 Flowchart of module E9 <\/td>\n<\/tr>\n | ||||||
211<\/td>\n | A.4 Description of clean-up (C) modules A.4.1 Module C0: No clean-up A.4.1.1 Principle A.4.1.2 Procedure A.4.1.3 Calculation <\/td>\n<\/tr>\n | ||||||
212<\/td>\n | A.4.1.4 Flowchart of module C0 A.4.2 Module C1: Clean-up by freezing-out of co-extracted fat, wax, sugars A.4.2.1 Principle A.4.2.2 Procedure A.4.2.3 Calculation <\/td>\n<\/tr>\n | ||||||
213<\/td>\n | A.4.2.4 Flowchart of module C1 A.4.3 Module C2: Clean-up by dispersive SPE with amine sorbent (PSA) A.4.3.1 Principle A.4.3.2 Procedure <\/td>\n<\/tr>\n | ||||||
214<\/td>\n | A.4.3.3 Calculation A.4.3.4 Flowchart of module C2 A.4.4 Module C3: Clean-up by dispersive SPE with a larger amount of amine sorbent (PSA) a) 50 mg PSA\/ml extract or b) 75 mg PSA\/ml extract) A.4.4.1 Principle <\/td>\n<\/tr>\n | ||||||
215<\/td>\n | A.4.4.2 Procedure A.4.4.3 Calculation <\/td>\n<\/tr>\n | ||||||
216<\/td>\n | A.4.4.4 Flowchart of module C3 A.4.5 Module C4: Clean-up by dispersive SPE with a mixture of amine sorbent (PSA) and silica based reverse phase sorbent (C18) A.4.5.1 Principle A.4.5.2 Procedure A.4.5.3 Calculation <\/td>\n<\/tr>\n | ||||||
217<\/td>\n | A.4.5.4 Flowchart of module C4 A.4.6 Module C5: Clean-up by dispersive SPE with a mixture of amine sorbent (PSA) and graphitized carbon black A.4.6.1 Principle <\/td>\n<\/tr>\n | ||||||
218<\/td>\n | A.4.6.2 Procedure A.4.6.3 Calculation <\/td>\n<\/tr>\n | ||||||
219<\/td>\n | A.4.6.4 Flowchart of module C5 A.5 Description of extract stabilization (S) modules A.5.1 Module S0: No extract stabilization A.5.1.1 Principle A.5.1.2 Procedure A.5.1.3 Flowchart of module S0 A.5.2 Module S1: Extract stabilization with formic acid A.5.2.1 Principle A.5.2.2 Procedure <\/td>\n<\/tr>\n | ||||||
220<\/td>\n | A.5.2.3 Flowchart of module S1 A.6 Description of detection (D) modules A.6.1 Module D1: Determination using liquid chromatography with tandem-mass spectrometric detection (LC-MS\/MS) A.6.1.1 Principle A.6.1.2 Procedure A.6.1.3 Apparatus A.6.1.4 Examples of suitable conditions <\/td>\n<\/tr>\n | ||||||
223<\/td>\n | A.6.1.5 Procedural remarks <\/td>\n<\/tr>\n | ||||||
224<\/td>\n | A.6.2 Module D2: Determination using liquid chromatography with accurate mass spectrometric detection (LC-accurate mass) A.6.2.1 Principle A.6.2.2 Procedure A.6.2.3 Apparatus A.6.2.4 Examples of suitable conditions <\/td>\n<\/tr>\n | ||||||
228<\/td>\n | A.6.2.5 Procedural remarks <\/td>\n<\/tr>\n | ||||||
229<\/td>\n | A.6.3 Module D3: Determination using gas chromatography with tandem-mass spectrometric detection (GC-MS\/MS) A.6.3.1 Principle A.6.3.2 Procedure A.6.3.3 Apparatus A.6.3.4 Examples of suitable conditions <\/td>\n<\/tr>\n | ||||||
231<\/td>\n | A.6.3.5 Procedural remarks <\/td>\n<\/tr>\n | ||||||
232<\/td>\n | A.6.4 Module D4: Determination using gas chromatography with mass spectrometric detection (GC-MS) A.6.4.1 Principle A.6.4.2 Procedure A.6.4.3 Apparatus <\/td>\n<\/tr>\n | ||||||
233<\/td>\n | A.6.4.4 Examples of suitable conditions <\/td>\n<\/tr>\n | ||||||
234<\/td>\n | A.6.4.5 Procedural remarks A.6.5 Module D5: Determination using gas chromatography with flame photometric detection (GC-FPD) A.6.5.1 Principle A.6.5.2 Procedure <\/td>\n<\/tr>\n | ||||||
235<\/td>\n | A.6.5.3 Apparatus A.6.5.4 Examples of suitable conditions <\/td>\n<\/tr>\n | ||||||
236<\/td>\n | A.6.6 Module D6: Determination using gas chromatography with electron capture detection (GC-ECD) A.6.6.1 Principle A.6.6.2 Procedure A.6.6.3 Apparatus A.6.6.4 Examples of suitable conditions <\/td>\n<\/tr>\n | ||||||
238<\/td>\n | A.7 Description of quantification (Q) options A.7.1 Option Q1: Quantification using external standards in solvent A.7.1.1 Principle A.7.1.2 Procedure A.7.1.3 Calculation A.7.2 Option Q2: Quantification using external standards in matrix A.7.2.1 Principle A.7.2.2 Procedure <\/td>\n<\/tr>\n | ||||||
239<\/td>\n | A.7.2.3 Calculation A.7.3 Option Q3: Quantification using a procedural internal standard and standards in solvent A.7.3.1 Principle A.7.3.2 Procedure A.7.3.3 Calculation A.7.4 Option Q4: Quantification using standard addition to the final extract A.7.4.1 Principle A.7.4.2 Procedure A.7.4.3 Calculation <\/td>\n<\/tr>\n | ||||||
240<\/td>\n | A.7.5 Option Q5: Quantification using a procedural internal standard and standards in matrix or isotope-labelled internal standards A.7.5.1 Principle A.7.5.2 Procedure A.7.5.3 Calculation A.7.6 Option Q6: Quantification using standard addition to the sample A.7.6.1 Principle <\/td>\n<\/tr>\n | ||||||
241<\/td>\n | A.7.6.2 Procedure A.7.6.3 Calculation A.7.7 Option Q7: Quantification by calibration of the entire procedure A.7.7.1 Principle A.7.7.2 Procedure A.7.7.3 Calculation <\/td>\n<\/tr>\n | ||||||
242<\/td>\n | Annex B (informative)Complementary information B.1 General B.2 Preparation of the buffer-salt mixture (A.1.8) B.3 Reagents for clean-up B.4 Prolongation of extraction time B.5 Working without internal standards B.6 Scaling <\/td>\n<\/tr>\n | ||||||
243<\/td>\n | B.7 Adjustment of pH value B.8 Recovery studies B.9 Clean-up with GCB B.10 Concentration of the final extracts and solvent exchange <\/td>\n<\/tr>\n | ||||||
244<\/td>\n | Annex C (informative)Abbreviations <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Tracked Changes. Foods of plant origin. Multimethod for the determination of pesticide residues using GC- and LC-based analysis following acetonitrile extraction\/partitioning and clean-up by dispersive SPE. Modular QuEChERS-method<\/b><\/p>\n |