{"id":338712,"date":"2024-10-19T23:42:21","date_gmt":"2024-10-19T23:42:21","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-234432020\/"},"modified":"2024-10-25T22:48:42","modified_gmt":"2024-10-25T22:48:42","slug":"bs-iso-234432020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-234432020\/","title":{"rendered":"BS ISO 23443:2020"},"content":{"rendered":"

This document specifies a method for the quantitative determination of ?-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. The application range runs from 1 ?g\/100 g to 1 500 ?g\/100 g for lycopene and from 1 ?g\/100 g to 2 250 ?g\/100 g for ?-carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1 ?g\/100 g and the limit of quantification (LOQ) was 0,3 ?g\/100 g for each carotenoid.<\/p>\n

The method does not apply to materials that contain measurable levels of ?-apo-8?-carotenal. The reproducibility data meets the requirements given in References [8<\/span>] and [10<\/span>].<\/p>\n

Annex C<\/span> specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference [9<\/span>].<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Principle
5 Reagents and materials
5.1 Reagents <\/td>\n<\/tr>\n
11<\/td>\n5.2 Standards <\/td>\n<\/tr>\n
12<\/td>\n5.3 Standards preparation <\/td>\n<\/tr>\n
14<\/td>\n6 Apparatus <\/td>\n<\/tr>\n
15<\/td>\n7 Procedure
7.1 Sample preparation <\/td>\n<\/tr>\n
17<\/td>\n7.2 Chromatography
7.2.1 Chromatographic conditions
7.2.2 System suitability checks <\/td>\n<\/tr>\n
18<\/td>\n8 Calculations
8.1 Determination of purity
8.1.1 General
8.1.2 Spectrophotometric purity (PS)
8.1.3 Chromatographic purity (PC) <\/td>\n<\/tr>\n
19<\/td>\n8.1.4 Reference standard purity (P)
8.2 Concentration of each carotenoid in standard solutions
8.2.1 Stock solution concentrations
8.2.2 Apocarotenal working solution concentration
8.2.3 Apocarotenal intermediate solution concentration <\/td>\n<\/tr>\n
20<\/td>\n8.2.4 Carotenoid concentrations in mixed carotenoid intermediate solution
8.2.5 Concentrations of carotenoid analytes in calibrations solutions
8.2.6 Concentration of apocarotenal internal standard in calibrations solutions
8.3 Calculate calibration curve <\/td>\n<\/tr>\n
21<\/td>\n8.4 Mass of apocarotenal
8.5 Contents of all-trans-\u03b2-carotene, cis isomers of \u03b2-carotene and total \u03b2-carotene <\/td>\n<\/tr>\n
22<\/td>\n8.6 Contents of total lycopene <\/td>\n<\/tr>\n
23<\/td>\n9 Precision
9.1 General
9.2 Repeatability
9.3 Reproducibility
10 Test report <\/td>\n<\/tr>\n
25<\/td>\nAnnex A (informative) Example chromatograms <\/td>\n<\/tr>\n
30<\/td>\nAnnex B (informative) Precision data <\/td>\n<\/tr>\n
33<\/td>\nAnnex C (informative) Determination of lutein <\/td>\n<\/tr>\n
40<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Infant formula and adult nutritionals. Determination of \u03b2-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2020<\/td>\n42<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338720,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338712","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338712","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338720"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338712"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338712"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}