BSI PD ISO/TS 21569-3:2015

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Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products – Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms

Published By	Publication Date	Number of Pages
BSI	2015	18

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Description

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PDF Pages	PDF Title
6	Foreword
7	Section sec_1 Section sec_2 Section sec_3 Section sec_4 1 Scope 2 Normative references 3 Terms and definitions 4 Principle
8	Section sec_5 Section sec_5.1 Section sec_5.2 Section sec_5.2.1 Section sec_5.2.2 Section sec_5.2.3 Table tab_1 Section sec_5.2.4 5 Reagents and materials 5.1 General 5.2 PCR reagents
9	Section sec_6 Section sec_6.1 Section sec_7 Section sec_7.1 Section sec_7.2 Section sec_7.3 Table tab_2 6 Apparatus 7 Procedure 7.1 Test sample preparation 7.2 Preparation of the DNA extracts 7.3 PCR setup
10	Section sec_7.4 Table tab_3 Section sec_8 Section sec_8.1 Section sec_8.2 Section sec_8.3 7.4 Temperature-time programme 8 Accept/reject criteria 8.1 General 8.2 Identification 8.3 Calculation of P35S-pat copy numbers
11	Section sec_9 Section sec_9.1 Section sec_9.2 Table tab_4 Table tab_5 9 Validation status and performance criteria 9.1 Robustness of the method 9.2 Collaborative trial for determination of LOD
12	Section sec_9.3 Table tab_6 9.3 Collaborative trial for quantification of the P35S-pat construct in rapeseed
13	Table tab_7 Section sec_9.4 Section sec_9.5 9.4 Sensitivity 9.5 Specificity
14	Section sec_10 10 Test report
15	Reference ref_1 Reference ref_2 Reference ref_3 Reference ref_4 Reference ref_5 Reference ref_6 Reference ref_7 Reference ref_8 Reference ref_9 Reference ref_10 Bibliography

Additional information

Status	Withdrawn
Title	Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products – Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms
Publisher	BSI
Committee	AW/275
Pages	18
Publication Date	2015-01-31
Withdrawn Date	2020-07-08
Replaced By	PD ISO/TS 21569-3:2020
ISBN	978 0 580 85097 4
Standard Number	PD ISO/TS 21569-3:2015
Identical National Standard Of	ISO/TS 21569-3:2015
Descriptors	Reproducibility, Biochemical methods of analysis, Food testing, Nucleic acids, Food products, Contaminant determination (food), Chemical analysis and testing, Genetically modified organisms, Qualitative analysis, Test equipment, Deoxyribonucleic acid
ICS Codes	67.050 - General methods of tests and analysis for food products

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BSI PD ISO/TS 21569-3:2015

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