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BS ISO 23732:2021

BS ISO 23732:2021

$142.49

Marine technology. Marine environment impact assessment (MEIA). General protocol for observation of meiofaunal community

Published By Publication Date Number of Pages
BSI 2021 28
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This document specifies a general protocol for the observation of the meiofaunal community in the deep seabed.

The standardized method can be used in any phase [baseline data acquisition, monitoring during and after mining (testing)] accompanying resource development, making it easier to compare data beyond differences in workers and waters.

This document is intended for marine environment impact assessments and other occasions where long-term image-based data are required.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Sampling
5.1 Method and equipment
11 5.2 Sub-sampling
5.3 Sample preservation
5.3.1 Preservation method before analysis
5.3.2 Preservation method after analysis
5.4 Recording sample information
6 Procedures for imaging flow cytometry (morphological analysis)
6.1 Pre-processing of samples
6.2 Observation using imaging flow cytometer
6.3 Image processing
12 7 Procedures for metagenomic analysis (molecular analysis)
7.1 General
7.2 Nucleic acids (DNA/RNA) extraction
7.3 PCR primers
7.4 PCR protocol
7.4.1 DNA
7.4.2 RNA (cDNA library production)
7.5 Check and purification of PCR amplicon
13 7.6 Index PCR
7.7 Purification of amplicon by magnetic beads
7.8 Concentration measurement
7.9 Quality check for amplicon by an automated electrophoresis system using microfluidic chip
7.10 Sequencing by a next generation sequencer
7.11 Data processing
8 Data and sample management
9 Combination of morphological analysis and molecular analysis (integrated analysis)
15 Annex A (informative) Example procedure for the analysis of the meiofaunal community by imaging flow cytometry
18 Annex B (informative) Example procedure for analysis of meiofaunal community by NGS
21 Annex C (informative) Example procedure for quantitative analysis of meiofaunal community by NGS
24 Annex D (informative) Useful information for users
25 Bibliography

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BS ISO 23732:2021
$142.49