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BS ISO 23443:2020

BS ISO 23443:2020

$167.15

Infant formula and adult nutritionals. Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)

Published By Publication Date Number of Pages
BSI 2020 42
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This document specifies a method for the quantitative determination of ?-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. The application range runs from 1 ?g/100 g to 1 500 ?g/100 g for lycopene and from 1 ?g/100 g to 2 250 ?g/100 g for ?-carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1 ?g/100 g and the limit of quantification (LOQ) was 0,3 ?g/100 g for each carotenoid.

The method does not apply to materials that contain measurable levels of ?-apo-8?-carotenal. The reproducibility data meets the requirements given in References [8] and [10].

Annex C specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference [9].

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Reagents and materials
5.1 Reagents
11 5.2 Standards
12 5.3 Standards preparation
14 6 Apparatus
15 7 Procedure
7.1 Sample preparation
17 7.2 Chromatography
7.2.1 Chromatographic conditions
7.2.2 System suitability checks
18 8 Calculations
8.1 Determination of purity
8.1.1 General
8.1.2 Spectrophotometric purity (PS)
8.1.3 Chromatographic purity (PC)
19 8.1.4 Reference standard purity (P)
8.2 Concentration of each carotenoid in standard solutions
8.2.1 Stock solution concentrations
8.2.2 Apocarotenal working solution concentration
8.2.3 Apocarotenal intermediate solution concentration
20 8.2.4 Carotenoid concentrations in mixed carotenoid intermediate solution
8.2.5 Concentrations of carotenoid analytes in calibrations solutions
8.2.6 Concentration of apocarotenal internal standard in calibrations solutions
8.3 Calculate calibration curve
21 8.4 Mass of apocarotenal
8.5 Contents of all-trans-β-carotene, cis isomers of β-carotene and total β-carotene
22 8.6 Contents of total lycopene
23 9 Precision
9.1 General
9.2 Repeatability
9.3 Reproducibility
10 Test report
25 Annex A (informative) Example chromatograms
30 Annex B (informative) Precision data
33 Annex C (informative) Determination of lutein
40 Bibliography

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BS ISO 23443:2020
$167.15