BS ISO 21702:2019
$142.49
Measurement of antiviral activity on plastics and other non-porous surfaces
| Published By | Publication Date | Number of Pages |
| BSI | 2019 | 30 |
This document specifies proper methods for measuring antiviral activity on plastics and other non-porous surfaces of antiviral-treated products against specified viruses. Due to the individual sensitivities, the results of one test virus might not be applicable for other viruses.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 7 | Foreword |
| 8 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Materials 4.1 Virus and host cell to be used for the tests 4.2 Reagents 4.3 Culture medium and solutions 4.3.1 General 4.3.2 Eagle’s minimum essential medium (EMEM) 4.3.3 RPMI 1640 medium |
| 11 | 4.3.4 7,5 % sodium bicarbonate solution 4.3.5 Formaldehyde solution 4.3.6 Methylene blue solution 4.3.7 Inactivated fetal bovine serum (FBS) 4.3.8 Growth medium 4.3.9 Maintenance medium |
| 12 | 4.3.10 Double concentration of the maintenance medium 4.3.11 Phosphate buffered saline [PBS (-)] 4.3.12 Trypsin derived from beef pancreas and PBS (-) solution 4.3.13 Trypsin EDTA solution 4.3.14 DEAE-dextran solution |
| 13 | 4.3.15 Agar medium for plaque assay 4.3.16 Soybean casein digest broth with lecithin and polyoxyethylene sorbitan monooleate (SCDLP broth) 5 Apparatus |
| 14 | 5.21 Test tubes. 5.22 Centrifuge tube. 5.23 Gauze or absorbent cotton. 6 Preparation 6.1 Restoration of host cell from cryopreservation |
| 15 | 6.2 Subculture of host cell 6.3 Cell culture for measuring virus infectivity titer 6.4 Preparation of test inoculums 6.4.1 Influenza virus |
| 16 | 6.4.2 Feline calicivirus 6.5 Preparation of test specimens |
| 17 | 6.6 Control test 6.6.1 General 6.6.2 Verification of cytotoxic effect on host cell 6.6.3 Verification of cell sensitivity to virus and the inactivation of antiviral activity |
| 19 | 7 Test procedure 7.1 Preparation of test specimen 7.2 Inoculation of test specimens |
| 20 | 7.3 Contact of the inoculated test specimens 7.4 Recovery of virus from test specimens 7.4.1 Test specimens immediately after inoculation 7.4.2 Test specimens after contact 7.5 Determining the infectivity titer of virus by plaque assay |
| 21 | 8 Expression of results 8.1 Determination of the infectivity titer of virus 8.2 Conditions for a valid test |
| 22 | 8.3 Calculation of the antiviral activity 8.4 Effectiveness of the antiviral agent 9 Repeatability and reproducibility 10 Test report |
| 24 | Annex A (informative) Composition of media |
| 26 | Annex B (informative) Repeatability and reproducibility |
| 28 | Bibliography |
