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BS EN ISO 23266:2021

$167.15

Soil quality. Test for measuring the inhibition of reproduction in oribatid mites (Oppia nitens) exposed to contaminants in soil

Published By Publication Date Number of Pages
BSI 2021 40
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This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and individual chemical substances on the reproduction of the oribatid mite Oppia nitens by dermal and alimentary uptake. This chronic (28-day) test is applicable to soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials). This method is not intended to replace the earthworm or Collembola tests since it represents another taxonomic group (= mites; i.e., arachnids), nor the predatory mite test since this species represents a different trophic level and ecological niche.

Effects of substances are assessed using standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil with similar properties to the soil sample to be tested (reference soil) or a standard soil (e.g., artificial soil).

Information is provided on how to use this method for testing substances under temperate conditions.

This document is not applicable to substances for which the air/soil partition coefficient is greater than 1, or to substances with vapour pressure exceeding 300 Pa at 25 °C.

NOTE The stability of the test substance cannot be assured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test. 

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
10 3 Terms and definitions
12 4 Principle
5 Reagents and material
15 6 Apparatus
7 Procedure
7.1 Experimental design
7.1.1 General
7.1.2 Range-finding test (preliminary test)
16 7.1.3 Definitive test
7.2 Preparation of test mixture
7.2.1 Testing contaminated soil
17 7.2.2 Testing substances added to the test substrate
18 7.2.3 Preparation of control containers
7.3 Addition of the mites
7.4 Test conditions and measurements
7.5 Determination of adult survival and reproductive output
19 8 Calculation and expression of results
8.1 Calculation
8.2 Expression of results
9 Validity of the test
20 10 Statistical analysis
10.1 General
10.2 Single-concentration tests
10.3 Multi-concentration tests
10.3.1 Range-finding test
10.3.2 Definitive test
21 11 Test report
23 Annex A (informative) Techniques for rearing and breeding of Oppia nitens
26 Annex B (normative) Determination of water holding capacity
27 Annex C (informative) Guidance on adjustment of pH of artificial soil
28 Annex D (informative) Extraction and counting of Oppia nitens
32 Annex E (informative) Performance of the method
37 Bibliography
BS EN ISO 23266:2021
$167.15