BS EN ISO 20418-3:2020
$142.49
Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres – Peptide detection using LC-MS without protein reduction
Published By | Publication Date | Number of Pages |
BSI | 2020 | 32 |
This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.
The composition of non-animal hair fibres can be measured by ISO 1833 (all parts) . Both results are combined to determine the total fibre composition.
The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827[ 4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present.
In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts) ].
PDF Catalog
PDF Pages | PDF Title |
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2 | undefined |
4 | European foreword Endorsement notice |
6 | Foreword |
7 | Introduction |
8 | 1 Scope 2 Normative references 3 Terms and definitions |
9 | 4 Symbols and abbreviated terms 5 Principle |
10 | 6 Reagents 7 Apparatus |
11 | 8 Test method 8.1 Sampling 8.2 Preliminary identification 8.3 Wash for degreasing 8.4 Powderization of fibres 8.5 Trypsin digestion |
12 | 8.6 Marker peptides 8.7 LC-MS analysis 8.8 Evaluation of the validity of observed data 8.9 Calculation of correction factor 8.9.1 Correction factor |
13 | 8.9.2 Correction factor for Cas1 against She1 and Yak1 (kc) 8.9.3 Correction factor for Yak2 against She2, She3 and Cas2 (ky) 8.9.4 Correction factor for alpaca and camel (ka) 8.9.5 Correction factor for angora (kr) 8.10 Calculation of blending ratio 8.10.1 Calculation of blending ratio of cashmere, sheep wool and yak |
14 | 8.10.2 Calculation by She1, Cas1, Yak1 and kc 8.10.3 Calculation by She2, She3, Cas2, Yak2 and ky 8.10.4 Calculation by She1, She2, She3, Cas2, Yak1 |
15 | 8.10.5 Calculation of blending ratio of camel, alpaca and angora |
16 | 9 Test report |
17 | Annex A (informative) Marker peptides of cashmere, sheep wool and yak fibres |
20 | Annex B (informative) Example of LC-MS analysis conditions |
23 | Annex C (informative) Analysis of camel, alpaca and angora fibres |
26 | Annex D (informative) Example of marker peptide mass chromatograms |
30 | Annex E (informative) Results of interlaboratory study |
31 | Bibliography |