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BS EN ISO 18416:2015:2017 Edition

BS EN ISO 18416:2015:2017 Edition

$142.49

Cosmetics. Microbiology. Detection of Candida albicans

Published By Publication Date Number of Pages
BSI 2017 28
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This International Standard gives general guidelines for the detection and identification of the specified microorganism Candida albicans in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621 ) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.

The method described in this International Standard is based on the detection of Candida albicans in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required.

NOTE

For the detection of Candida albicans, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).

Because of the large variety of cosmetic products within this field of application, this method may not be appropriate in every detail for some products (e.g. certain water immiscible products). Other International Standards ( ISO 18415 ) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.

PDF Catalog

PDF Pages PDF Title
4 European foreword
Endorsement notice
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Diluents and culture media
5.1 General
11 5.2 Diluent for the yeast suspension (tryptone sodium chloride solution)
5.2.1 General
5.2.2 Composition
5.2.3 Preparation
5.3 Culture media
5.3.1 General
5.3.2 Agar medium for suitability test (see Clause 11)
12 5.3.3 Enrichment broth
13 5.3.4 Selective agar medium for isolation of Candida albicans
5.3.5 Corn meal agar with 1 % polysorbate 80
6 Apparatus and glassware
7 Strains of microorganisms
14 8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension in the enrichment broth
9.2.1 General
9.2.2 Water-miscible products
15 9.2.3 Water-immiscible products
9.2.4 Filterable products
9.3 Incubation of the inoculated enrichment broth
9.4 Detection and identification of Candida albicans
9.4.1 Isolation
9.4.2 Identification of Candida albicans
16 10 Expression of the results (detection of Candida albicans)
11 Neutralization of the antimicrobial properties of the product
11.1 General
11.2 Preparation of inoculum
17 11.3 Suitability of the detection method
11.3.1 Procedure
11.3.2 Interpretation of suitability test results
12 Test report
19 Annex A (informative) Other media
24 Annex B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
25 Bibliography

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BS EN ISO 18416:2015
$142.49