BS EN ISO 16654:2001+A2:2023
$167.15
Microbiology of food and animal feeding stuffs. Horizontal method for the detection of Escherichia coli O157. Inclusion of performance testing of all culture media and reagent
| Published By | Publication Date | Number of Pages |
| BSI | 2023 | 34 |
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | undefined |
| 4 | European Foreword Anchor 3 European Foreword to amendment A1 |
| 5 | Blank Page |
| 6 | Annex ZA (normative) Normative references to international publications with their relevant European publications |
| 7 | Blank Page |
| 10 | Foreword |
| 11 | Introduction |
| 13 | 1 Scope 2 Normative references 3 Term and definition |
| 14 | 4 Principle 5 Culture media, reagents and antisera 5.1 Enrichment medium: Modified tryptone soya broth with novobiocin (mTSB + N) 5.1.1 Modified tryptone soya broth (mTSB) 5.1.2 Novobiocin solution |
| 15 | Blank Page |
| 16 | 5.2 First selective isolation medium: Cefixime tellurite sorbitol MacConkey agar (CT-SMAC) 5.2.1 Base medium 5.2.2 Potassium tellurite solution |
| 17 | 5.2.3 Cefixime solution 5.2.4 Complete medium 5.3 Second selective isolation medium |
| 18 | 5.4 Nutrient agar 5.4.1 Composition 5.4.2 Preparation 5.4.3 Preparation of nutrient agar plates 5.5 Tryptone/tryptophan medium 5.5.1 Composition 5.5.2 Preparation |
| 19 | 5.6 Kovac’s indole reagent 5.6.1 Composition 5.6.2 Preparation 5.7 Anti-Escherichia coli O157 immunomagnetic particles 5.8 Wash buffer: Modified phosphate buffer, 0,01mol/l, of pH 7,2 5.8.1 Composition 5.8.2 Preparation 5.9 Normal saline solution 5.9.1 Composition |
| 20 | Blank Page |
| 21 | 5.9.2 Preparation 6 Apparatus and glassware 6.1 Apparatus for dry sterilization (oven) and/or wet sterilization (autoclave) |
| 22 | 6.16 Vortex mixer 7 Sampling 8 Preparation of test sample 9 Procedure (see annex A) 9.1 Test portion and initial suspension 9.2 Enrichment |
| 23 | 9.3 Immunomagnetic separation (IMS) 9.3.1 General 9.3.2 Immunocapture 9.3.3 Separation 9.4 Plating out onto selective agars and identification of E. coli O157 colonies 9.4.1 Plating out |
| 24 | 9.4.2 Recognition of typical E. coli O157 colonies 9.5 Confirmation 9.5.1 Selection of colonies 9.5.2 Biochemical confirmation: Indole formation 9.5.3 Serological identification |
| 25 | 9.6 Further characterization 10 Quality assurance 10.1 Test strains for quality assurance purposes 10.2 Culture method 11 Expression of results 12 Performance characteristics of the method 12.1 Interlaboratory study 12.2 Sensitivity |
| 26 | 12.3 Specificity 12.4 LOD50 13 Test report |
| 27 | Annex A (normative) Diagram of procedure |
| 28 | Annex B (informative) Results of interlaboratory studies |
| 31 | Bibliography |
