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BS EN ISO 11816-2:2024 – TC

BS EN ISO 11816-2:2024 – TC

$186.33

Tracked Changes. Milk and milk products. Determination of alkaline phosphatase activity – Fluorimetric method for cheese

Published By Publication Date Number of Pages
BSI 2024 56
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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in cheese. This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7). The instrument used for the determination of ALP can read activities in the supernatant up to 7 000 milliunits per litre (mU/kg).

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PDF Pages PDF Title
32 undefined
43 9.1 Verification of instrument performance
9.1.1 General
9.1.2 Daily instrument tests
9.1.3 Using FLM200
9.1.4 Using FLM300
44 9.1.5 Controls
9.2 Reagent controls to test the suitability of ready-to-use working substrate (5.3)
9.3 Calibration
9.3.1 General
45 9.3.2 Using FLM200
9.3.3 Using FLM300
9.4 Determination
46 9.5 Test-sample-related controls
9.5.1 Recommended negative and positive control tests
47 9.5.2 Interfering substance test
9.5.3 Heat-stable microbial ALP control test
10.1 Calibration ratio
48 10.2 Calculation
10.2.1 Supernatant
10.2.2 Cheese
10.3 Expression of test results
49 11.1 Interlaboratory study
11.2 Repeatability
11.3 Reproducibility

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BS EN ISO 11816-2:2024 - TC
$186.33