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BS EN ISO 11816-1:2024

BS EN ISO 11816-1:2024

$142.49

Milk and milk products. Determination of alkaline phosphatase activity – Fluorimetric method for milk and milk-based drinks

Published By Publication Date Number of Pages
BSI 2024 24
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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution. The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not higher than 7 000 mU/l.

PDF Catalog

PDF Pages PDF Title
2 undefined
12 8.1 Alkaline phosphatase-free milk
8.2 Preparation of the test sample
8.2.1 General
8.2.2 Pasteurized test samples
8.2.3 Dilution of test samples with high ALP values
9.1 Verification of instrument performance
9.1.1 General
13 9.1.2 Daily instrument tests
9.1.3 Using FLM200
9.1.4 Using FLM300
9.1.5 Controls
14 9.2 Reagent controls to test the suitability of ready-to-use working substrate (5.3)
9.3 Calibration
9.3.1 General
9.3.2 Using FLM200
9.3.3 Using FLM300
15 9.4 Determination
16 9.5 Test-sample-related controls
9.5.1 Recommended negative and positive control tests
9.5.2 Interfering substance test
9.5.3 Heat-stable microbial alkaline phosphatase control test
10.1 Calibration ratio
17 10.2 Calculation
10.3 Expression of test results
11.1 Interlaboratory study
11.2 Repeatability
11.3 Reproducibility

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BS EN ISO 11816-1:2024
$142.49