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BS EN ISO 11290-1:2017

BS EN ISO 11290-1:2017

$189.07

Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Detection method

Published By Publication Date Number of Pages
BSI 2017 50
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This document specifies a horizontal method for

  • the detection of L. monocytogenes, and

  • the detection of Listeria spp. (including L. monocytogenes).

This document is applicable to

  • products intended for human consumption and for the feeding of animals, and

  • environmental samples in the area of food production and food handling.

It is possible that certain additionally described Listeria species may not be detected or confirmed by this method.[5],[10],[12],[14],[25],[26],[27]

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
9 Foreword
10 Introduction
11 1 Scope
2 Normative references
12 3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth)
13 4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.4 Plating out and identification
4.5 Confirmation
5 Culture media and reagents
6 Equipment and consumables
14 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment
15 9.3 Secondary enrichment
9.4 Plating out and identification
9.4.1 General
9.4.2 Agar Listeria according to Ottaviani and Agosti
16 9.4.3 Second selective medium
9.5 Confirmation of Listeria monocytogenes or Listeria spp.
9.5.1 Selection of colonies for confirmation
9.5.2 Confirmation of L. monocytogenes
20 9.5.3 Confirmation of Listeria spp.
21 9.6 Interpretation of morphological and physiological properties and of the biochemical reactions
9.7 Additional characterization of isolated strains (optional)
10 Expression of results
11 Performance characteristics of the method
11.1 Method validation studies
11.2 Sensitivity
11.3 Specificity
11.4 Level of detection (LOD50)
22 12 Test report
13 Quality assurance
23 Annex A (normative) Diagram of procedure
24 Annex B (normative) Composition and preparation of culture media and reagents
37 Annex C (informative) Distinction of Listeria spp. from other genera
38 Annex D (informative) Reactions for the identification of Listeria species
40 Annex E (informative) Listeria selective agars
41 Annex F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes
45 Bibliography

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BS EN ISO 11290-1:2017
$189.07