BS EN ISO 10272-1:2017 – TC:2020 Edition
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Tracked Changes. Microbiology of the food chain. Horizontal method for detection and enumeration of Campylobacter spp. – Detection method
Published By | Publication Date | Number of Pages |
BSI | 2020 | 80 |
PDF Catalog
PDF Pages | PDF Title |
---|---|
46 | National foreword |
51 | Foreword |
52 | Introduction |
53 | 1 Scope 2 Normative references 3 Terms and definitions |
54 | 4 Principle 4.1 General 4.2 Enrichment in selective liquid medium 4.2.1 Detection procedure A 4.2.2 Detection procedure B 4.2.3 Detection procedure C |
55 | 4.3 Isolation on selective solid medium 4.3.1 Detection procedure A 4.3.2 Detection procedure B 4.3.3 Detection procedure C 4.3.4 Detection procedure A, B and C 4.4 Confirmation 5 Culture media and reagents 6 Equipment and consumables |
56 | 7 Sampling 8 Preparation of test sample 9 Procedure 9.1 General |
57 | 9.2 Test portion and initial suspension 9.2.1 General 9.2.2 Detection procedure A 9.2.3 Detection procedure B 9.2.4 Detection procedure C |
58 | 9.3 Enrichment 9.3.1 Detection procedure A 9.3.2 Detection procedure B 9.4 Isolation 9.4.1 Detection procedure A 9.4.2 Detection procedure B 9.4.3 Detection procedures A, B and C 9.5 Confirmation of Campylobacter 9.5.1 General |
59 | 9.5.2 Selection of colonies for confirmation 9.5.3 Examination of morphology and motility 9.5.4 Study of aerobic growth at 25 °C 9.5.5 Detection of oxidase activity 9.5.6 Interpretation |
60 | 9.6 Identification of Campylobacter species (optional) 9.6.1 General 9.6.2 Detection of catalase activity 9.6.3 Detection of hippurate hydrolysis 9.6.4 Detection of indoxyl acetate hydrolysis |
61 | 9.6.5 Interpretation 10 Expression of results 11 Performance characteristics of the method 11.1 Interlaboratory study 11.2 Sensitivity 11.3 Specificity 11.4 LOD50 |
62 | 12 Test report |
63 | Annex A (normative) Diagram of procedures |
64 | Annex B (normative) Culture media and reagents |
73 | Annex C (informative) Method validation studies and performance characteristics |
76 | Bibliography |