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BS EN ISO 10272-1:2017 – TC:2020 Edition

$217.84

Tracked Changes. Microbiology of the food chain. Horizontal method for detection and enumeration of Campylobacter spp. – Detection method

Published By Publication Date Number of Pages
BSI 2020 80
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PDF Catalog

PDF Pages PDF Title
46 National foreword
51 Foreword
52 Introduction
53 1 Scope
2 Normative references
3 Terms and definitions
54 4 Principle
4.1 General
4.2 Enrichment in selective liquid medium
4.2.1 Detection procedure A
4.2.2 Detection procedure B
4.2.3 Detection procedure C
55 4.3 Isolation on selective solid medium
4.3.1 Detection procedure A
4.3.2 Detection procedure B
4.3.3 Detection procedure C
4.3.4 Detection procedure A, B and C
4.4 Confirmation
5 Culture media and reagents
6 Equipment and consumables
56 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
57 9.2 Test portion and initial suspension
9.2.1 General
9.2.2 Detection procedure A
9.2.3 Detection procedure B
9.2.4 Detection procedure C
58 9.3 Enrichment
9.3.1 Detection procedure A
9.3.2 Detection procedure B
9.4 Isolation
9.4.1 Detection procedure A
9.4.2 Detection procedure B
9.4.3 Detection procedures A, B and C
9.5 Confirmation of Campylobacter
9.5.1 General
59 9.5.2 Selection of colonies for confirmation
9.5.3 Examination of morphology and motility
9.5.4 Study of aerobic growth at 25 °C
9.5.5 Detection of oxidase activity
9.5.6 Interpretation
60 9.6 Identification of Campylobacter species (optional)
9.6.1 General
9.6.2 Detection of catalase activity
9.6.3 Detection of hippurate hydrolysis
9.6.4 Detection of indoxyl acetate hydrolysis
61 9.6.5 Interpretation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
62 12 Test report
63 Annex A (normative) Diagram of procedures
64 Annex B (normative) Culture media and reagents
73 Annex C (informative) Method validation studies and performance characteristics
76 Bibliography
BS EN ISO 10272-1:2017 - TC
$217.84