🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BS EN 14176:2017

BS EN 14176:2017

$102.76

Foodstuffs. Determination of domoic acid in raw shellfish, raw finfish and cooked mussels by RP-HPLC using UV detection

Published By Publication Date Number of Pages
BSI 2017 22
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Categories: ,

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

This European Standard specifies methods for the quantitative determination of domoic acid in raw bivalve molluscs and finfish as well as in cooked mussels. The limit of detection is about 10 ng/ml to 80 ng/ml (0,05 mg/kg to 0,4 mg/kg), depending on the UV detector sensitivity. The limit of quantification for DA by these methods is at least 2,7 mg/kg. Method A has been validated for the determination of DA in different raw matrices such as mussels, clams, scallops and anchovies, spiked and/or naturally contaminated at levels ranging from 2,7 mg/kg to 85,1 mg/kg. Method B has been validated for the determination of DA at levels ranging from 5 mg/kg to 12,9 mg/kg in cooked blue mussels. For further information on validation data, see Clause 8 and Annex A. Laboratory experience has shown that this standard can also be applied to other shellfish species, however, no complete validation study according to ISO 5725 has been carried out so far.

PDF Catalog

PDF Pages PDF Title
7 1 Scope
2 Normative references
3 Principle
4 Reagents
9 5 Apparatus
10 6 Procedure
6.1 Sample preparation
6.1.1 Raw bivalves with shell
6.1.2 Raw bivalves without shell (whole body or any edible part separately)
6.1.3 Raw fish
11 6.1.4 Cooked mussels)
6.2 Extraction procedure
6.2.1 Method A
6.2.2 Method B
6.2.3 Centrifugation, dilution and storage
6.3 HPLC determination
6.3.1 General
12 6.3.2 HPLC-conditions
6.4 Calibration graph
6.5 Sample injection
13 7 Evaluation of results
7.1 Identification
7.2 Quantification
8 Precision
14 9 Test report
15 Annex A (informative)Precision data
A.1 Precision data for Method A using isocratic elution
16 A.2 Precision data for Method B using gradient elution
17 A.3 Precision data from EURLMB Proficiency Testing Schemes
18 Annex B (informative)Typical chromatogram

Related products

BS EN 14176:2017
$102.76