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BS EN 14122:2014

BS EN 14122:2014

$142.49

Foodstuffs. Determination of vitamin B1 by high performance liquid chromatography

Published By Publication Date Number of Pages
BSI 2014 26
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This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig’s liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives.

For further information on the validation, see Clause 8 and Annex B.

PDF Catalog

PDF Pages PDF Title
5 Foreword
6 1 Scope
2 Normative references
3 Principle
4 Reagents
9 5 Apparatus
10 6 Procedure
6.1 Preparation of the test sample
6.2 Preparation of the sample test solution
6.2.1 Extraction
6.2.2 Enzyme treatment
6.2.3 Sample test solution
6.3 Oxidation of thiamin to thiochrome
6.3.1 General
11 6.3.2 Pre-column oxidation
6.3.2.1 Procedure for oxidation step
6.3.2.2 Identification with HPLC after pre-column oxidation
6.3.3 Post-column oxidation
6.3.3.1 Procedure for oxidation step
6.3.3.2 Identification with HPLC using post-column oxidation
12 6.4 Determination
7 Calculation
13 8 Precision
8.1 General
8.2 Repeatability
8.3 Reproducibility
14 9 Test report
15 Annex A (informative) Examples of HPLC chromatograms
Figure A.1 — Example for an HPLC separation of thiamin as thiochrome standard using pre-column derivatization
16 Figure A.2 — Examples for a HPLC separation of thiamin using post-column derivatization in lettuce (a), cooked rice (b) and cooked pork meat (c)
17 Annex B (informative) Precision data
Table B.1 — Precision data for whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig’s liver
18 Table B.2 — Precision data for tube feeding solution, baby food with vegetables, powdered milk, meal with fruits and yeast
19 Table B.3 — Precision data for cereal, chocolate powder and food supplement
20 Annex C (informative) Alternative HPLC systems
Table C.1 — Alternative HPLC conditions
21 Annex D (informative) Vitamin B1 compound: 2-(1-hydroxyethyl)thiamin (HET) performing post-column derivatization
22 Figure D.1 — Chromatogram of an extract of CRM 487 pigs liver (a) and a standard of 0,1 µg/ml TMP (Thiamin Mono Phosphate), 0,1 µg/ml thiamin and 0,01 µg/ml HET (2-(1-hydroxyethyl)thiamin) (b)
23 Bibliography

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BS EN 14122:2014
$142.49