ASTM-E2839 2011
$40.63
E2839-11 Standard Test Method for Production of Clostridium difficile Spores for Use in Efficacy Evaluation of Antimicrobial Agents
Published By | Publication Date | Number of Pages |
ASTM | 2011 | 5 |
ASTM E2839-11
Historical Standard: Standard Test Method for Production of Clostridium difficile Spores for Use in Efficacy Evaluation of Antimicrobial Agents
ASTM E2839
Scope
1.1 This test method is for producing C. difficile spores to evaluate antimicrobial formulations for their sporicidal activity.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them when appropriate.
1.3 This standard may involve hazardous materials, chemicals, and microorganisms and should be performed only by persons with formal training in microbiology.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Keywords
acid resistance; Clostridium difficile; density gradient medium; spore production; spore purity; sporicidal efficacy testing; vegetative cells
ICS Code
ICS Number Code 07.100.99 (Other standards related to microbiology)
DOI: 10.1520/E2839-11
PDF Catalog
PDF Pages | PDF Title |
---|---|
1 | Scope Terminology Summary of Test Method |
2 | Significance and Use Apparatus Media and Reagents Test Organism Hazards |
3 | Preparation of Frozen Stock Cultures of Test Organism Preparation of a Test Spore Suspension from FSC |
4 | Spore Purification Quantitative Acid Resistance Test Calculation |
5 | Precision and Bias Keywords REFERENCES |