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ASTM-E2839 2011

$40.63

E2839-11 Standard Test Method for Production of Clostridium difficile Spores for Use in Efficacy Evaluation of Antimicrobial Agents

Published By Publication Date Number of Pages
ASTM 2011 5
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ASTM E2839-11

Historical Standard: Standard Test Method for Production of Clostridium difficile Spores for Use in Efficacy Evaluation of Antimicrobial Agents

ASTM E2839

Scope

1.1 This test method is for producing C. difficile spores to evaluate antimicrobial formulations for their sporicidal activity.

1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them when appropriate.

1.3 This standard may involve hazardous materials, chemicals, and microorganisms and should be performed only by persons with formal training in microbiology.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

acid resistance; Clostridium difficile; density gradient medium; spore production; spore purity; sporicidal efficacy testing; vegetative cells

ICS Code

ICS Number Code 07.100.99 (Other standards related to microbiology)

DOI: 10.1520/E2839-11

PDF Catalog

PDF Pages PDF Title
1 Scope
Terminology
Summary of Test Method
2 Significance and Use
Apparatus
Media and Reagents
Test Organism
Hazards
3 Preparation of Frozen Stock Cultures of Test Organism
Preparation of a Test Spore Suspension from FSC
4 Spore Purification
Quantitative Acid Resistance Test
Calculation
5 Precision and Bias
Keywords
REFERENCES
ASTM-E2839 2011
$40.63