ASTM-E1510:2013 Edition

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E1510-95(2013)e1 Standard Practice for Installing Fused Silica Open Tubular Capillary Columns in Gas Chromatographs

Published By	Publication Date	Number of Pages
ASTM	2013	13

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Categories: 71.040.50 - Physicochemical methods of analysis, ASTM

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Description

ASTM E1510-95-e1-Reapproved2013

Historical Standard: Standard Practice for Installing Fused Silica Open Tubular Capillary Columns in Gas Chromatographs

ASTM E1510

Scope

1.1 This practice covers the installation and maintenance of fused silica capillary columns in gas chromatographs that are already retrofitted for their use. This practice excludes information on:

1.1.1 Injection techniques.

1.1.2 Column selection.

1.1.3 Data acquisition.

1.1.4 System troubleshooting and maintenance.

1.2 For additional information on gas chromatography, please refer to Practice E260. For specific precautions, see 7.2.2.2(1), 7.2.2.2(2), 7.2.7, and 7.2.7.2.

1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific safety information, see Section 6, 7.2.2.2(1), 7.2.2.2(2), 7.2.7, and 7.2.7.2.²

TABLE 1 Typical Splitter Vent Flow Rates (50 to 1 split ratio) (at optimum linear velocity)

Note 1—The curves were generated by plotting the height equivalent to a theoretical plate (length of column divided by the total number of theoretical plates, H.E.T.P.) against the column’s average linear velocity. The lowest point on the curve indicates the carrier gas velocity in which the highest column efficiency is reached.

Note 2—Gases information available from Compressed Gas Association (CGA), 4221 Walney Rd., 5th Floor, Chantilly, VA 20151-2923, http://www.cganet.com.

FIG. 1 Van Deemter Profile for Hydrogen, Helium, and Nitrogen Carrier Gases

Note 1—Fig. 2 shows that the resolution is similar but the analysis time is reduced by 50 % when comparing hydrogen to helium in an isothermal analysis using optimum flow velocities.

Note 2—Hydrogen provides similar resolution in one-half the analysis time of helium for an isothermal analysis.

Note 3—

Note 4—30 m, 0.25-mm ID, 0.25 μm 5 % diphenyl − 95 % dimethyl polysiloxane 0.1-μL split injection of chlorinated pesticides.

FIG. 2 Hydrogen Versus Helium (Isothermal Analysis)

FIG. 3 Capping Silanol Groups with Dimethyl Dichlorosilane (DMDCS)

Note 1—Septum bleed can obscure or co-elute with compounds of interest, thus decreasing the analytical accuracy.

Note 2—

Note 3—30 m, 0.53-mm ID, 0.50 μm 5 % diphenyl − 95 % dimethyl polysiloxane 0.1 μL direct injection of 50 pg pesticide standard.

FIG. 4 ECD Septum Bleed

Keywords

capillary columns; capillary gas chromatography; gas chromatographs; installing capillary columns

ICS Code

ICS Number Code 71.040.50 (Physicochemical methods of analysis)

DOI: 10.1520/E1510-95R13E01

Additional information

Linear velocity: 40 cm/s	Linear velocity: 20 cm/s

Linear velocity: 40 cm/s (Flow rate: 10 cm 3 /min)
Carrier gas: Hydrogen	Carrier gas: Helium
Injector temperature: 250°C	Detector temperature: 300°C
Oven temperature: 150 to 275°C at 4°C/min, hold 15 min
helium hydrogen	35 70
ECD sensitivity: 8 × 10 −11 AFS
Carrier gas	0.25-mm ID, cm3 /min
Carrier gas: Helium
ICS Codes	71.040.50 - Physicochemical methods of analysis