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ASTM-D7687 2011

$40.63

D7687-11 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration

Published By Publication Date Number of Pages
ASTM 2011 9
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ASTM D7687-11

Historical Standard: Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration

ASTM D7687

Scope

1.1 This test method covers a protocol for capturing, extracting and quantifying the cellular adenosine triphosphate (cellular-ATP) content associated with microorganisms found in fuels, fuel/water mixtures and fuel-associated water.

1.2 The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of cellular-ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard, computation to pg ATP/mL and optional further transformation to Log10[pg ATP/mL].

1.3 This test method is equally suitable for use as a laboratory or portable method.

1.4 This test method is limited to fuels with a nominal viscosity 75cSt at test temperature.

1.5 This test method detects ATP concentrations in the range of 5.0 pg ATP/mL (0.699 log10[pg ATP/mL]) to 100 000 pg ATP/mL (5.000 log10[pg ATP/mL]) for 20 mL samples of fuel or fuel/water mixtures, and 20 pg ATP/mL (1.301 log10[pg ATP/mL]) to 400 000 pg ATP/mL (5.602 log10[pg ATP/mL]) for 5 mL samples of fuel-associated water.

Note 1—These ranges were calculated with the formula for calculating sample ATP in pg/mL provided in 12.1 based on the minimum recommended RLU for a 1 ng ATP/mL standard when using the reagents specified in Section 7 and the luminometer specified in 6.4 and corrected with a reagent-method blank as determined in Appendix X5.

1.6 Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. This test method does not differentiate between ATP from different sources, for example: from different types of microorganisms, such as bacteria and fungi.

1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

adenosine triphosphate; ATP; bacteria; bioburden; biodeterioration; biodiesel; biofuels; biomass; cellular; cellular-ATP; diesel; fuel; fuel-oil; fungi; gasoline; microbial contamination; microbiology; microorganisms

ICS Code

ICS Number Code 75.160.20 (Liquid fuels)

DOI: 10.1520/D7687-11

PDF Catalog

PDF Pages PDF Title
1 Scope
Referenced Documents
2 Terminology
Summary of Test Method
Significance and Use
3 Apparatus
Reagents and Materials
4 Hazards
Sampling, Test Specimens, and Test Units
Calibration and Standardization
Procedure
5 Calculation of Results
Precision and Bias
6 Keywords
X1. RELATIONSHIP BETWEEN ATP CONCENTRATION AND POPULATION DENSITY
X1.1
X1.2
X1.3
X1.4
X2. ATP STANDARD CURVE METHOD
X2.1
X2.2
X2.3
7 X3. ADJUSTING METHOD DETECTION RANGE
X3.1 Increasing Test Sensitivity (Increasing RLUobs by Filtration)
X3.2 Decreasing Test Sensitivity (Decreasing RLUobs by Dilution)
X4. EVALUATION OF ASSAY INTERFERENCES
X4.1
X4.2 Uncontaminated Sample Evaluation by Standard Addition Method
TABLE X4.1
TABLE X4.2
8 X4.3 Contaminated Sample Evaluation by Dilution Series Method
X5. DETERMINATION OF BACKGROUND RLU
X5.1
X5.2
X5.3
X6. PRELIMINARY INTERLABORATORY STUDY TO ESTABLISH REPEATABILITY FOR MEASUREMENT OF CELLULAR ATP IN FUELS AND FUEL-ASSOCIATED WATER WITH SAMPLE CONCENTRATION BY FILTRATION
X6.1
X6.2 The following laboratory participated in this ILS
X6.3 Description of Samples
X6.4 ILS Instructions
TABLE X6.1
9 X6.5 Description of Equipment/Apparatus
X6.6
X6.7 Statistical Data Summary
X6.8 Preliminary Repeatability Statement:
TABLE X6.2
ASTM-D7687 2011
$40.63